Author(s)

Ruiming Fan¹, Yu Wei², LiLi Wu², YuanWen Liu^2, Lei Gong^3*

Affiliation(s)

¹Cerebrovascular Department, Affiliated Hospital of Zunyi Medical University, Zunyi 563099, Guizhou, China.

²Pharmacy Department, Affiliated Hospital of Zunyi Medical University, Zunyi 563099, Guizhou, China.

³Guizhou Engineering Research Center for Translational Medicine, Zunyi Medical University, Zunyi 563099, Guizhou, China.

Corresponding Author

Lei Gong

ABSTRACT

Objective: To investigate the intervention effect of OPN (osteopontin) on the expression of nuclear factor-KB (Fume-xappab, NF-KB) and interleukin-1 (IL-1) after hyperglycemic cerebral hemorrhage in rats. Methods: The hyperglycemia cerebral hemorrhage model was made by autologous blood injection. The neurological function behavior score was performed by Garcia method in each group. The changes of brain water content were measured by wet weight method. Results: The expression of NF-KB and IL-1 protein was significantly increased after hyperglycemia cerebral hemorrhage, the expression of NF-KB and IL-1 protein was significantly decreased after osteopontin intervention, and the brain water content was significantly decreased, which was consistent with the cerebral nerve dysfunction score. Conclusion: Osteopontin may inhibit the expression of IL-1 through the NF-KB pathway, reduce the inflammatory damage of the brain tissue around the hematoma after hyperglycemia cerebral hemorrhage, and play a protective role on the brain tissue.

KEYWORDS

Hyperglycemia cerebral hemorrhage; Osteopontin; Nuclear factor -KB; Interleukin 1 beta

CITE THIS PAPER

Ruiming Fan, Yu Wei, LiLi Wu, YuanWen Liu, Lei Gong. OPN (osteopontin) regulates neuroinflammation in rats with hyperglycemic cerebral hemorrhage. Journal of Pharmaceutical and Medical Research. 2024, 6(3): 16-20. DOI: 10.61784/jpmr3010.

REFERENCES

[1] Chen Jinsong, Li Feng, Bai Jie, et al. The effect of rat nerve growth factor on the treatment of neurological deficit in elderly patients with cerebral hemorrhage and its influence on the level of inflammatory factors. Chinese Journal of Physicians, 2018,20 (12): 1869-1871.

[2] Lei G, Anatol M, Ruiming F, et al. Osteopontin attenuates inflammation via JAK2/STAT1 pathway in hyperglycemic rats after intracerebral hemorrhage. Neuropharmacology, 2018, 138:160-169.

[3] Liang Qiong, Xiang Wang, Zou Jia, et al. Effect of Zhuanggu Zhitong Capsule on regulating OSX and OPN proteins on osteoblast differentiation in ovariectomized rats. Journal of Hunan University of Traditional Chinese Medicine, 2019, 39 (03): 29-33.

[4] Xing Yanyang, Li Zhongnan, Xing Yuting, et al. Effect of Jianpi Recipe on renal osteopontin and renal pathology of diabetic rats. Chinese Journal of Clinical Health, 2018, 21(02): 225-229.

[5] Xiang Shaotong, Xiao Hao, Chen Anne. The value of serum osteopontin level in the diagnosis and prognosis evaluation of patients with acute cerebral hemorrhage. Chongqing Medical Journal, 2017, 34 (46): 92-95.

[6] Changchun H, Ping L, Xiao Y, et al.Inhibition of mTOR signaling Confers Protection against Cerebral Ischemic Injury in Acute Hyperglycemic Rats. International Journal of Biological Sciences, 2017, 13(7): 878-887.

[7] Gu Jianwei, Li Yun, Yang Yunfeng, et al. Effects of active vitamin D3 on airway inflammation and osteopontin expression in cough variant asthma rats. Journal of Hainan Medical College, 2020, 026 (010): 754-759.

[8] Mostafa D G, Satti H H, Khaleel E F, et al.A high-fat diet rich in corn oil exaggerates the infarct size and memory impairment in rats with cerebral ischemia and is associated with suppressing osteopontin and Akt, and activating GS3Kβ, iNOS, and NF-κB. Journal of Physiology and Biochemistry, 2020, 76(3):393-406.

[9] Zhao Shijiao, Han Xue, Gao Yanjun. Prospective study on the correlation between plasma osteopontin and fibrinogen levels and the degree of neurological impairment and prognosis in patients with acute cerebral infarction. Journal of Practical Cardio cerebral Pulmonary Vascular Diseases, 2018, 26(008): 32-36

[10] Jianyun L, Zhijin S, Caishan H .Comparison of osteopontin levels inpatients with cerebral hemorrhage of different ages. The Medical Forum, 2018.

[11] Pang Chunyan, Zhang Wei, Liu Yuan, et al. Therapeutic effect of small interfering RNA targeting osteopontin on lupus nephritis in MRL/Lpr mice. Journal of Immunology, 2018, 034(005): 393-400

[12] Liu Ting, Li Dan, Zhang Yan, et al. Study on the predictive value of osteopontin and inflammatory factors in the progress of mild cognitive impairment. China General Practice, 2018, 21(32): 30-34+42

[13] A M F D A A, A F K D A B, Mayron Alves de Vasconcelos a b c, et al. Antihyperglycemic and antioxidant activities of a lectin from the marine red algae, Bryothamnion seaforthii, in rats with streptozotocin-induced diabetes. International Journal of Biological Macromolecules, 2020, 158:773-780.

[14] Yang Xinxin, Hu Zhenzhu, Wang Yuqiao, et al. Study on the correlation between osteopontin and non motor symptoms of Parkinson's disease. Stroke and Neuropathy, 2017 (6): 489-491.

[15] Yang Pingping, Zhang Wei, Chi Lijun. New progress in molecular mechanism of osteopontin in immune related diseases. Journal of Immunology, 2017, 033(003): 263-267.