THE RESEARCH PROGRESSION OF SEROLOGICAL ANALYSIS OF RECOMBINANT cDNA EXPRESSION LIBRARIES SCREENING ANTIGENS OF ATHEROSCLEROTIC DISEASES

Abstract

The serological analysis of recombinant cDNA expression libraries (SEREX) is an important research method of screening disease-associated antigens, it combines the gene cloning technology with the serum screening technology, the recombinant cDNA expression clone library is screened by autologous serum of patients, thus the relevant target antigen genes are obtained, In turn, serum antibodies of patients can be identified by the target genes. It was initially used to screen tumor-related antigens, then the range of application expanded and gradually applied to screen the antigens of atherosclerotic disease. This article reviews the basic principles, experimental procedures, advantages and disadvantages of SEREX and its application and research progress in screening antigens of atherosclerotic disease.