MECHANISM STUDY OF SHENFU INJECTION REGULATING MAPK PATHWAY TO ALLEVIATE MYOCARDIAL ISCHEMIA-REPERFUSION INJURY IN RATS BASED ON BIOINFORMATICS AND EXPERIMENTAL VERIFICATION

Authors

  • Hong Wang Department of Cardiology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang 330006, Jiangxi, China
  • Liu Yang Department of Cardiology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang 330006, Jiangxi, China
  • HuaiGang Chen Medical College of Nanchang University, Nanchang 330006, Jiangxi, China
  • GaoMing Hong The People’s Hospital of Yujiang District, Yingtan 335000, Jiangxi, China
  • ShengQiang Zeng Department of Cardiology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang 330006, Jiangxi, China
  • WenYan Xu Department of Cardiology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang 330006, Jiangxi, China , Medical College of Nanchang University, Nanchang 330006, Jiangxi, China
  • Yao Hu (Corresponding Author) Department of Cardiology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang 330006, Jiangxi, China

Keywords:

Shenfu Injection (SFI), Myocardial Ischemia-Reperfusion Injury (MIRI), Mitogen-Activated Protein Kinase (MAPK) signaling pathway, Bioinformatics, Inflammatory factors

Abstract

To elucidate SFI’s protective mechanism against MIRI, this study identified the MAPK pathway via bioinformatics. Experimental validation in MIRI rats confirmed that SFI improves myocardial injury by suppressing MAPK activation and inflammatory factors (IL-1β, IL-6, TNF-α), supporting its clinical potential.

References

[1] Zhao C, Liu T, Wei H, et al. Serum Oxidative Stress Factors Predict Myocardial Ischemia Reperfusion Injury after Percutaneous Coronary Intervention in Patients with Acute Myocardial Infarction and Type 2 Diabetes Mellitus. Postepy Kardiol Interwencyjnej, 2023, 19(04): 333-342.

[2] Zhou Y, Liang Q, Wu X, et al. siRNA Delivery against Myocardial Ischemia Reperfusion Injury Mediated by Reversibly Camouflaged Biomimetic Nanocomplexes. Advanced Materials, 2023, 35(23): e2210691.

[3] Huang P, Wu SP, Wang N, et al. Hydroxysafflor Yellow A Alleviates Cerebral Ischemia Reperfusion Injury by Suppressing Apoptosis via Mitochondrial Permeability Transition Pore. Phytomedicine, 2021, 85(01): 153532.

[4] Lin H, Xiong W, Fu L, et al. Damage-Associated Molecular Patterns (DAMPs) in Diseases: Implications for Therapy. Molecular Biomedicine, 2025, 6(01): 60.

[5] Shi Y, Han B, Yu X, et al. Ginsenoside Rb3 Ameliorates Myocardial Ischemia-Reperfusion Injury in Rats. Pharmaceutical Biology, 2011, 49(09): 900-906.

[6] Yang W, Bai X, Tang Y, et al. miR-379-3p Activates the MAPK/JNK/p38 Pathway by Negatively Targeting DUSP1 Expression to Promote Myocardial Ischemia-Reperfusion Injury. Clinical Hemorheology and Microcirculation, 2025, 90(02): 73-83.

[7] Shi Y, Xu S, Ngoi NYL, et al. PRL-3 Dephosphorylates p38 MAPK to Promote Cell Survival under Stress. Free Radical Biology and Medicine, 2021, 177(01): 72-87.

[8] Zhang L, Jiang Y, Jia W, et al. Modelling Myocardial Ischemia/Reperfusion Injury with Inflammatory Response in Human Ventricular Cardiac Organoids. Cell Proliferation, 2025, 58(03): e13762.

[9] Prem PN, Sivakumar B, Boovarahan SR, et al. Recent Advances in Potential of Fisetin in the Management of Myocardial Ischemia-Reperfusion Injury-A Systematic Review. Phytomedicine, 2022, 101(01): 154123.

[10] Liu X, Ai F, Li H, et al. Anti-Inflammatory Effects of Shenfu Injection against Acute Lung Injury through Inhibiting HMGB1-NF-κB Pathway in a Rat Model of Endotoxin Shock. Evidence-Based Complementary and Alternative Medicine, 2019(01), 9857683.

[11] Qin X, Liu B, Gao F, et al. Gluconolactone Alleviates Myocardial Ischemia/Reperfusion Injury and Arrhythmias via Activating PKCε/Extracellular Signal-Regulated Kinase Signaling. Front Physiol, 2022, 13(01): 856699.

[12] Negm S, Wolf M, Craveiro RB, et al. Resveratrol Modulates Phosphorylation of ERK and AKT in Murine Cementoblasts during in Vitro Orthodontic Compression. BMC Oral Health, 2025, 25(01): 226.

[13] Zhao L, Wu X, Li Q, et al. Inhibition of CCN5 Protects Against Apoptosis and Endoplasmic Reticulum Stress in Bisphenol A-Induced Sertoli Cells via p38/JNK MAPK Signaling Pathway. DNA and Cell Biology, 2025, 44(04): 174-185.

[14] Song D, Liu Y, Yao Y, et al. Melatonin improves bisphenol A-induced cell apoptosis, oxidative stress and autophagy impairment via inhibition of the p38 MAPK signaling pathway in FLK-BLV cells. Environmental Toxicology, 2022, 37(7): 1551-1562.

[15] Doganyigit Z, Eroglu E, Akyuz E. Inflammatory Mediators of Cytokines and Chemokines in Sepsis: From Bench to Bedside. Human & Experimental Toxicology, 2022, 41(01): 9603271221078871.

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Published

2025-11-14

Issue

Vol. 7 No. 3 (2025)

Section

Research Article

DOI:

https://doi.org/10.61784/jpmr3048

How to Cite

Wang, H., Yang, L., Chen, H., Hong, G., Zeng, S., Xu, W., Hu, Y. (2025). Mechanism Study Of Shenfu Injection Regulating Mapk Pathway To Alleviate Myocardial Ischemia-Reperfusion Injury In Rats Based On Bioinformatics And Experimental Verification. Eurasia Journal of Science and Technology, 7(3), 15-19. https://doi.org/10.61784/jpmr3048