Author(s)

Jingxian Fang^1*, Dan Li², Baoyi Xie¹, Hedi Liu¹, Fei Ren¹

Affiliation(s)

¹Department of Pediatric dentistry, Stomatological Hospital, Southern Medical  University, Guangzhou, 510280, China

²Department of stomatology, Guangzhou Women and Children's Medical Center,  Guangzhou, 510623, China

Corresponding Author

Jingxian Fang

ABSTRACT

Purpose: To explore the possibility of applying Guilin watermelon frost  in pulp revascularization of young permanent teeth with periapical periodontitis.  Material and methods: Divided the stem cells from human exfoliated deciduous  teeth (SHED) into A. B. C and D four groups. A without any treatment, B. C. D groups were treated with calcium hydroxide, triantibiotic intracanal dressing and Guilin watermelon frost separately for one hour after treatment of conventional  irrigation of 1% sodium hypochlorite and normal saline. Results: Compared to group A, cell growth was found to be slower down in group B. C. and D. No statistic differences were found. While after special treatment, cell survival rate decreased significantly, group D decreased more than group C, and group B survived more than group C. Conclusion: Guilin watermelon frost is not fit for pulp revascularization of immature permanent teeth with periapical periodontitis temporarily.

KEYWORDS

Watermelon Frost; Stem cells from human exfoliated deciduous teeth  (SHED); Periapical diseases in immature permanent teeth

CITE THIS PAPER

Jingxian Fang, Dan Li, Baoyi Xie, Hedi Liu, Fei Ren. Exploration of application of Guilin watermelon frost in pulp revascularization of immature permanent teeth with periapical periodontitis. Acta Translational Medicine. 2020, 3(1): 1-7.

REFERENCES

[1]. Wigler R, Kaufman AY, Lin S, et al. Revascularization: a treatment for permanent teeth with necrotic pulp and incomplete root development. J Endod 2013;39(3):319-26. DOI: 10.1016/j.joen.2012.11.014

[2]. da Silva LA, Nelson-Filho P, da Silva RA, et al. Revascularization and periapical repair after endodontic treatment using apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing in dogs' teeth with apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109(5):779-87. DOI: 10.1016/j.tripleo.2009.12.046

[3]. Yun Ren XL, Jianguo Min. Determination of menthol in Guilin watermelon frost throat pain spray by gas chromatography (In Chinese). Electronic Journal of Clinical Medical Literature 2019;6(61):165. DOI: 10.16281/j.cnki.jocml.2019.61.147

[4]. Mei L. Determination of Baicalin in Guilin watermelon frost by RP-HPLC (In Chinese). Guangxi Medical Journal 2000;22(3):477-78.

[5]. Yang J. Determination of Berberine in Guilin xiguashuang Spray by HPLC (In Chinese). Research and Practice of Chinese Medicines 2004;18(2):34-35. DOI: 10.13728/j.1673-6427.2004.02.013

[6]. Khadijeh Karbalaie ST, Farzaneh Rabiei, Abbas Kiani-Esfahani, Najmeh Sadat Masoudi, Mohammad Hossein Nasr-Esfahani, and Hossein Baharvand. Stem Cells from Human Exfoliated Deciduous Tooth Exhibit Stromal-Derived Inducing Activity and Lead to Generation of Neural Crest Cells from Human Embryonic Stem Cells. Cell J 2015;17(1):37–48. DOI: 10.22074/cellj.2015.510

[7]. Yamagata M, Yamamoto A, Kako E, et al. Human dental pulp-derived stem cells protect against hypoxic-ischemic brain injury in neonatal mice. Stroke 2013;44(2):551-4. DOI: 10.1161/STROKEAHA.112.676759

[8]. Ishkitiev N, Yaegaki K, Imai T, et al. Novel management of acute or secondary biliary liver conditions using hepatically differentiated human dental pulp cells. Tissue Eng Part A 2015;21(3-4):586-93. DOI: 10.1089/ten.TEA.2014.0162

[9]. Aggarwal V, Singla M. Conventional apexification and revascularization induced maturogenesis of two non-vital, immature teeth in same patient: 24 months follow up of a case. J Conserv Dent 2012;15(1):68-72. DOI: 10.4103/0972-0707.92610

[10]. Chen MY, Chen KL, Chen CA, et al. Responses of immature permanent teeth with infected necrotic pulp tissue and apical periodontitis/abscess to revascularization procedures. Int Endod J 2012;45(3):294-305. DOI: 10.1111/j.1365-2591.2011.01978.x

[11]. Wang X, Thibodeau B, Trope M, et al. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod 2010;36(1):56-63. DOI: 10.1016/j.joen.2009.09.039

[12]. Zeng Q, Nguyen S, Zhang H, et al. Release of Growth Factors into Root Canal by Irrigations in Regenerative Endodontics. J Endod 2016;42(12):1760-66. DOI: 10.1016/j.joen.2016.04.029

[13]. Mohammadi Z, Jafarzadeh H, Shalavi S, et al. A Review on Triple Antibiotic Paste as a Suitable Material Used in Regenerative Endodontics. Iran Endod J 2018;13(1):1-6. DOI: 10.22037/iej.v13i1.17941