Author(s)

Dan Li¹, Zhenping Lin^1＃, Peng Qing^2*, Zhenwen Liang¹, Yu'en Huang¹, Jing Hu²

Affiliation(s)

¹The Rehabilitation Department of the First Affiliated Hospital of Jinan University, Guangzhou, 510630, China. 

²The Acu-moxibustion Department of the First Affiliated Hospital of Jinan University, Guangzhou, 510630, China.

Corresponding Author

Peng Qing

ABSTRACT

Objective: To discuss the effect of Electro-acupuncture (EA) on the expression of Orexin-A and C-fos in hypothalamus of sleep deprivation rats after cerebral ischemia-reperfusion injury. Methods: The cerebral ischemia-reperfusion injury (MCAO/R)models were established with reference to the modified longa thread bolt method. All MCAO/R models were divided into the MCAO/R model group, the control group and the experimental group. The sleep deprivation models were made by the method of soft stimulation combined with cage replacement. The rats in the experimental group were given EA intervention for 7 days, and the acupoints were Shenmen and Sanyinjiao. After the experiment, the content of Orexin-A and C-fos in hypothalamus was detected by the method of ELISA. Results: Compared with the blank group, the content of Orexin-A and C-fos of the other four groups increased significantly (P<0.05).Compared with the control group, the content of Orexin-A and C-fos of the experimental group, the MCAO/R model group and sleep deprivation model group decreased significantly(P<0.05).The content of Orexin-A of the MCAO/R model group was significantly lower than the sleep deprivation model group (P<0.05). The content of C-fos of the experimental group was significantly lower than the sleep deprivation model group (P<0.05). Conclusion ： The cerebral ischemia-reperfusion injury and sleep deprivation all can activate the expression of Orexin-A neurons and C-fos gene to a certain extent and affect the sleep-wake cycle of rat. EA may regulate the disordered sleep-wake cycle of sleep deprivation rat after cerebral ischemia-reperfusion injury by inhibiting the expression of Orexin-A neurons and C-fos gene.

KEYWORDS

Electro-acupuncture (EA); cerebral ischemia-reperfusion injury; Sleep Deprivation; Orexin-A; C-fos

CITE THIS PAPER

Dan Li, Zhenping Lin, Peng Qing, Zhenwen Liang, Yu'en Huang, Jing Hu. Effect of electro-acupuncture on the expression of orexin-a and c-fos in hypothalamus of sleep deprivation rats after cerebral ischemia-reperfusion injury. Acta Translational Medicine. 2020, 3(1): 8-14.

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