Author(s)

Yu Gu, Wenting Fu, Yonghua Yao, Xi Zheng^*

Affiliation(s)

Department of anesthesiology, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, 510030, China

Corresponding Author

Xi Zheng

ABSTRACT

Objective: To investigate the effects of dexmedetomidine (Dex) or propofol on oxidative stress and the activation of endoplasmic reticulum stress (ERS) in renal cells under hypoxia/reoxygenation (H/R) conditions. Methods: Human tubular epithelial cells (HK-2) were selected and divided into Control group, hypoxia 24h and reoxygenation24 (H24R4) group, Dex group and Propofol group. Cell growth was detected by CCK-8 assay kit, malondialdehyde (MDA) and superoxidase dismutase (SOD) were detected by kits, and the expressions of ERS-related key genes such as ER chaperone BiP (GRP78) and C/EBP homologous protein (CHOP) were detected by qPCR. Results: Compared with the Control group, the cell growth and SOD activity of HK-2 cells in H24R4 group were significantly decreased, while MDA content and the expressions of GRP78 and CHOP in these cells were significantly increased (P<0.05). Pretreatment with Dex or propofol could significantly improve the cell growth and SOD activity of H24R4-treated cells, and reduce intracellular MDA content and GRP78 expression (P<0.05). Dex also obviously alleviated CHOP expression in H24R4-treated cells (P<0.05). Conclusion:s Dex or propofol pretreatment could reduce oxidative stress and attenuates ERS activation in H24R4-treated cells. Dex had a stronger inhibitory effect on the activation of ERS-related apoptosis way compared with propofol.

KEYWORDS

Dexmedetomidine, Propofol, Hypoxia/Reoxygenation, Oxidative Stress, Endoplasmic Reticulum Stress

CITE THIS PAPER

Yu Gu, Wenting Fu, Yonghua Yao, Xi Zheng. Effect of dexmedetomidine or propofol on endoplasmic reticulum stress in HK-2 cells with hypoxia/reoxygenation. Acta Translational Medicine. 2020, 3(1): 62-66.

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