Author(s)

ChenWei Li^1#*, Ke Wang^2#, QiuXu Chen¹, Jie Yan¹

Affiliation(s)

¹Clinical Laboratory, The People’s Hospital of Yubei District,Chongqing.Yubei, Chongqing 401120, China.

²Clinical Laboratory, Yongchuan People's Hospital of Chongqing, Chongqing 402160, China.

Corresponding Author

ChenWei Li

ABSTRACT

Objective: The purpose of this study was to explore the expression of MRPL13 in breast carcinoma and normal breast tissues based on TCGA database, and to explore the influence of MRPL13 on the prognosis of breast carcinoma patients. Methods: Firstly, to analyze the expression of MRPL13 difference in breast carcinoma and normal tissue by TCGA. Secondly, to establish MRPL13 co-expression gene network by using String database and perform on MRPL13 expression spectrum data  GO analysis and KEGG analysis by making use of DAVID database. In the end, to analyze the total survival of breast cancer takes advantage of CBioPortal and kaplan-meie Plot. Results: The TCGA database showed that 43% of the 816 samples  up-regulated MRPL13 expression in breast cancer tissues. TO obtain interaction pairs of co-expressed MRPL13 genes from the String database. Biological functions main focus on regulating DNA recombination and repair, transcription initiation and binding of transcription factors, and assembly of protein macromolecular complexes, which mainly occurs in the cell membrane system. KEGG pathway analysis was mainly concentrated in cell division cycle and oxidative phosphorylation. The total survival rate (OS) of 1402 breast cancer patients and 814 breast cancer patients respectively via Kaplan-meier Plotter and cBioPortal . Conclusion: Over-expression of MRPL13 in breast cancer tissue predicts poor prognosis and it can regard as one of the prognostic markers of breast cancer patients.

KEYWORDS

Breast cancer; MRPL13; Prognosis; TCGA database

CITE THIS PAPER

ChenWei Li, Ke Wang, QiuXu Chen, Jie Yan. Expression of MRPL13 and its prognostic significance in breast cancer. World Journal of Biomedical Sciences. 2024, 2(2): 20-25. DOI: 10.61784/wjbs3002.

REFERENCES

[1] Zhong X, He Z, Fan Y, et al. Multi-omics analysis of MRPL-13 as a tumor-promoting marker from pan-cancer to lung adenocarcinoma. Aging (Albany NY), 2023, 15(19): 10640-10680.

[2] Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015. CA Cancer J Clin, 2016, 66(2): 115-132.

[3] Chen D, Nemazanyy I, Peulen O, et al. Elp3-mediated codon-dependent translation promotes mTORC2 activation and regulates macrophage polarization. EMBO J, 2022, 41(18): e109353.

[4] Lee YK, Lim JJ, Jeoun UW, et al. Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness. J Biol Chem, 2017, 292(49): 20208-20217.

[5] Lin X, Guo L, Lin X, et al. Expression and prognosis analysis of mitochondrial ribosomal protein family in breast cancer. Sci Rep, 2022, 12(1): 10658.

[6] Hillier LD, Lennon G, Becker M, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res, 1996, 6(9): 807-828.

[7] Brown A, Rathore S, Kimanius D, et al. Structures of the human mitochondrial ribosome in native states of assembly. Nat Struct Mol Biol, 2017, 24(10): 866-869.

[8] Lee YK, Lim JJ, Jeoun UW, et al. Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness. J Biol Chem, 2017, 292(49): 20208-20217.

[9] Gyorffy B, Lanczky A, Eklund AC, et al.An online survival analysis tool to rapidly assess the effect of 22, 277 genes on breast cancer prognosis using microarray data of 1809 patients. Breast Cancer Res Treatment, 2010, 123(3): 725-731.

[10] Miller KD, Siegel PL, Lin CC, et al. Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin, 2016, 66(4): 271-289.

[11] Tao Z, Suo H, Zhang L, et al. MRPL13 is a Prognostic Cancer Biomarker and Correlates with Immune Infiltrates in Breast Cancer. Onco Targets Ther, 2020, 13:12255-12268.

[12] Lopez J, Tait SW. Mitochondrial apoptosis: killing cancer using the enemy within. Br J Cancer, 2015, 112(6): 957-962.

[13] Lee YK, Youn HG, Wang HJ, et al. Decreased mitochondrial OGG1 expression is linked to mitochondrial defects and delayed hepatoma cell growth. Mol Cells, 2013, 35(6): 489-497.

[14] Liu Y, Sun H, Li X, et al. Identification of a Three-RNA Binding Proteins (RBPs) Signature Predicting Prognosis for Breast Cancer. Front Oncol, 2021, 11:663556.

[15] Ye H, Zhang N. Identification of the Upregulation of MRPL13 as a Novel Prognostic Marker Associated with Overall Survival Time and Immunotherapy Response in Breast Cancer. Comput Math Methods Med, 2021, 2021: 1498924.

[16] Greber BJ, Ban N. Structure and Function of the Mitochondrial Ribosome. Annu Rev Biochem, 2016, 85: 103-132.

[17] Gopisetty G, Thangarajan R. Mammalian mitochondrial ribosomal small subunit (MRPS) genes: A putative role in human disease. Gene, 2016, 589(1): 27-35.