Author(s)

JinFeng Hu^*, XianZhi Zhou, Lei Lin

Affiliation(s)

Fujian Engineering Research Center for Green Pest Management, Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, China.

Corresponding Author

JinFeng Hu

ABSTRACT

The green peach aphid Myzus persicae (Sulzer, 1776) (GPA) is an economic pest that damages many agricultural crops and has evolved resistance to various classes of insecticides, including neonicotinoids. Thiacloprid is a broad-spectrum neonicotinoid with low toxicity to bees. After fifty selection cycles from a susceptible strain (FAA-S), a strain (THG-R) with 1,201.2-fold resistance to thiacloprid was obtained under laboratory conditions. The THG-R strain exhibited a high level of cross-resistance to imidacloprid (894.3-fold), moderate levels of cross-resistance to acetamiprid (48.7-fold), dinotefuran (44.7-fold), flupyradifurone (24.9-fold), and sulfoxaflor (14.6-fold), and low levels of cross-resistance to thiamethoxam (9.4-fold) and clothianidin (6.4-fold). Synergism and activity tests indicated the possible involvement of cytochrome P450 in the detoxification resistance to thiacloprid. Transcriptome profiling of the THG-R and FAA-S strains identified 72 differentially expressed genes (DEGs) related to insecticide detoxification, including 15 upregulated and 57 downregulated genes. Resistance was not conferred by known detoxification mechanisms of resistance to neonicotinoid insecticides and sulfoxaflor, but rather by overexpression of the P450 genes CYP6CY3 and CYP380c40 and UDP-glucuronosyltransferase (UGT) gene UGT344P2. The adenosine triphosphate (ATP)-binding cassette transporters (ABCs) Mplethal(2)03659.1 and thioesterase MpThem6 were highly overexpressed (30.3-fold and 7-fold, respectively) in the THG-R strain. These findings provided another possible enhanced metabolic route responsible for neonicotinoid resistance in GPA.

KEYWORDS

Myzus persicae; Thiacloprid; Resistance development; Cross-resistance; Detoxification mechanism

CITE THIS PAPER

JinFeng Hu, XianZhi Zhou, Lei Lin. Transcriptome analysis of thiacloprid-resistant myzus persicae reveals the overexpression of metabolic detoxification genes. Eurasia Journal of Science and Technology. 2024, 6(5): 24-38. DOI: https://doi.org/10.61784/ejst3033.

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