Author(s)

Aloy-Amadi Oluchi C.^1*, Okoroafor Helen A.¹, Dimeke Chioma¹, Ukonu Uche C.¹, Chukwuigwe-Igbere Orokwu Eziaku², Akogu Okechukwu.³

Affiliation(s)

¹Department of Medical Laboratory Science, Imo State University, Owerri, Nigeria.

²Department of Haematology and Blood Transfusion, Faculty of Medical Laboratory Science, Rivers State University, Port- Harcourt, Nigeria.

³Department of Optometry, Imo State University, Owerri, Nigeria.

Corresponding Author

Aloy-Amadi Oluchi C.

ABSTRACT

Prostate cancer is the uncontrolled growth of cells in the prostate gland in males. It represents  the second most common cancer in men and the fifth most common cause of cancer death in men. This study was aimed at evaluating the levels of some haematological parameters, creatinine and urea in patients with prostate cancer on a three month treatment. It was a cross-sectional study carried out from the month of June to August, 2023. All eligible subjects who filled the questionnaire and gave a written informed consent for the study were enrolled. The study population consisted of 30 male patients with prostate cancer who were on treatment, and an equivalent number of age-matched apparently healthy subjects who served as the controls. 7ml of venous blood sample was collected at the ante-cubital vein aseptically. 2ml was dispensed into ethylenediaminetetraacetic acid containers for WBC and differential white cell counts, while 5ml was dispensed into plain containers for urea and creatinine estimations. The EDTA and plain containers were properly labeled with the subjects’ names, sample number and date of collection. The blood dispensed into the EDTA containers were stored in a refrigerator at 40C, while the serum was stored in a freezer at -200C prior to use. The procedure was carried out at the Specialist Hospital, Umuguma, Owerri. WBC and differential counts were determined using haematology autoanalyzer, urea was determined using the diacetyl monoxime method, while creatinine was determined using the alkaline picrate method. The results of the tests were analyzed using SPSS version 21. The mean values of WBC (8300.00±2419.03)cells/μl, Neutrophils (55.27±17.76)%, and Monocytes (11.87±4.38)% were significantly increased in prostate cancer patients when compared to controls (4943.33±1208.21)cells/μl, (40.10±7.04)  and (4.38±3.19)% (p=0.000). There were no significant difference in the mean values of lymphocytes (44.80±10.93)% and eosinophils (2.03±1.75)% in prostate cancer patients when compared to controls (44.83±64.93)% and (2.10±2.12)% (p=0.998 and 0.895). The mean values of serum creatinine (1.63±0.79)mg/dl and urea (40.90±28.43)mg/dl were significantly increased in prostate cancer patients when compared to controls (19.43±6.60)mg/dl and (0.72±0.34)mg/dl (p=0.000). There was no significant difference in the mean values of WBC, neutrophils, lymphocytes, monocytes, eosinophils, urea and creatinine in prostate cancer patients when compared based on age (p=0.476, p=0.624, p=0.621, p=0.471, p=0.228, p=0.358 and p=0.265). There was a non- significant positive correlation of WBC with urea and creatinine in prostate cancer patients (r=0.15, p=0.435 and r=0.14, p=0.439). Prostate cancer is associated with significant increase in the mean values of urea and creatinine. There were also alterations in the levels of total white blood cell, neutrophils and monocytes in prostate cancer patients. Therefore haematological parameters, urea and creatinine should be included into the panel of test for screening of a prostate cancer patients.

KEYWORDS

Urea; Creatinine; White blood cell; Differential white cell; Prostate cancer

CITE THIS PAPER

Aloy-Amadi Oluchi C., Okoroafor Helen A., Dimeke Chioma, Ukonu Uche C., Chukwuigwe-Igbere Orokwu Eziaku, Akogu Okechukwu. Studies on urea, creatinine, white blood cell and differential white cell counts in prostate cancer patients on treatment attending Imo state specialist hospital umuguma, owerri, Nigeria. Journal of Pharmaceutical and Medical Research. 2024, 6(4): 32-36. DOI: https://doi.org/10.61784/jpmr3022.

REFERENCES

[1] Aligbe JU. Morphological characterization of prostate diseases in adult males, a retrospective survey from UBTH: A dissertation submitted to the National Postgraduate Medical College of Nigeria. ResearchGate, 2015, 43(9): 73-90.

[2] McArdle PA, Mir K, Almushatat AS, et al. Systemic inflammatory response, prostatespecific antigen and survival in patients with metastatic prostate cancer. Urology Journal, 2016, 77: 127–129.

[3] Hoffman RM, Gilliland FD, Eley JW, et al. Racial and ethnic differences in advanced-stage prostate cancer: the Prostate Cancer Outcomes Study. Journal of National Cancer Institute, 2018, 93(5): 388–395.

[4] Montagnana M, Cervellin G, Meschi T et al. The role of haematological indices in cardiovascular and thrombotic disorders. Clinical Chemistry and Laboratory Medicine, 2016, 50: 635-641.

[5] Kautz L, Nemeth E. Molecular liaisons between erythropoiesis and iron metabolism. Blood, 2014, 124(4): 479–482.

[6] Baxmann AC, Ahmed MS, Marques NC. Influence of muscle mass physical activity on serum urinary creatinine serum cystatin. Clinical Journal of American Nephrology, 2018, 23: 348–354.

[7] Corbett S. Urinary beta 2-microglobulin and N-acetyl-beta-D-glucosaminidase (NAG) as early markers of renal tubular dysfunction in sick neonates. Journal of Formos Medical Association, 2018, 90(2): 132–137

[8] Lalitha K, Suman G, Pruthvish S, et al. Estimation of time trends of incidence of prostate cancer-an Indian scenario. Asian Pacific Journal of Cancer Preview, 2016, 13(12): 6245-6250.

[9] Rodrigues A, George W, Padraig T, et al. Pre-treatment risk stratification of prostate cancer patients. Canadian Urological Association Journal, 2016, 6(2): 121-127.

[10] Mansour SA, Mossa AH, Heikal TM. Haematoxicity  of  a  new  natural  insecticide Spinosad on male Albino rats. International  Journal of Agricultural Biology, 2017, 9: 342-346.

[11] De Nunzio C, Kramer G, Marberger M, et al.; The controversial relationship between benign prostatic hyperplasia and prostate cancer: the role of inflammation. Eur Urol, 2011, 60: 106-117.

[12] Fukami  A,  Yamagishi  S, Adachi  H. High white blood cell  count  and  low  estimated  glomerular filtration  rate  are  independently  associated with   serum   level   of   monocyte chemo-attractant pbrotein-1 in a general population. Clinical Cardiology, 2017, 34(3): 189-194.

[13] Ogunbiyi OJ. Impact of health system challenges on prostate cancer control: health care experiences in Nigeria. Infectious Agent Cancer, 2015, 12(3): 12-14.

[14] Düzlü M, Karamert R, Tutar H. Diagnostic role of neutrophil-lymphocyte ratio in oral cavity cancers. Journal of Clinical Practic, 2018, 21(1): 49-53.

[15] Feng Li, Haio Hu, Shuo Gu, et al. Platelet to lymphocyte ratio plays an important role in prostate cancers diagnosis and prognosis. International Journal of Clinical and Experimental Medicine, 2015, 8(7): 11746 – 117551.

[16] Abelev GI, Eraiser TL. Cellular aspects of alpha-fetoprotein expression in tumors. Seminar Cancer Biology, 2015, 9: 95-107.

[17] Merseburger AS, Connelly RR, Sun L, et al. Use of serum creatinine to predict pathologic stage and recurrence among radical prostatectomy patients. Urology, 2017, 58:729–34.